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Abstract Background Antibody-drug conjugates ADCs targeting the RON receptor, a tumorigenic factor contributing to cancer malignancy, has been considered as a novel strategy for cancer therapy. Here we describe a humanized antibody recognizing the RON plexin-semaphorin-integrin PSI domain with increased drug delivery capability for potential clinical application. Stability of H5Bbased ADCs in human Nude Nude Female Drug Represenatives Drug Reprrsenatives was measured using hydrophobic interaction chromatography.